Herpetomonas megaseliae: Architectural Rearrangements during Amastigote Formation

Herpetomonas megaseliae: architectural rearrangements during amastigote formation.

Herpetomonas megaseliae and Crithidia harmosa: growth on blood-agar plates.

A technique for culturing two species of trypanosomatid flagellates on blood-agar plates is described. Herpetomonas megaseliae and Crithidia harmosa were grown as discrete clone colonies on plates prepared in sterile petri dishes with a medium derived from that of Tobie et al. (1950, J Parasitol 36: 48-54). Plates were inoculated with 0.01 ml flagellate suspension and the inoculum was spread ev...

Sialoglycoconjugates in Herpetomonas megaseliae: role in the adhesion to insect host epithelial cells.

Herpetomonas megaseliae is a monoxenic trypanosomatid isolated from the phorid fly Megaselia scalaris. In the present report, the expression of cell surface sialoglycoconjugates in this parasite was analyzed by Western blotting, flow cytometry and fluorescence microscopy analyses using lectins that specifically recognize sialic acid residues. A strong reaction was detected when parasites were t...

Quick-freeze, deep-etch rotary replication of Trypanosoma cruzi and Herpetomonas megaseliae.

The fine structure of epimastigotes of Trypanosoma cruzi and promastigotes of Herpetomonas megaseliae was analysed in replicas of quick-frozen, freeze-fractured, deeply etched and rotary-replicated cells. Using control cells and cells treated with Triton X-100 before glutaraldehyde fixation, images were obtained that showed connections of the sub-pellicular microtubules with each other, with th...

Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote.

Differentiation of Trypanosoma cruzi trypomastigotes to amastigotes inside myoblasts or in vitro, at low extracellular pH, in the presence of [(3)H]palmitic acid or [(3)H]inositol revealed differential labeling of inositolphosphoceramide and phosphatidylinositol, suggesting that a remodeling process takes place in both lipids. Using (3)H-labeled inositolphosphoceramide and phosphatidylinositol ...